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KMID : 0384119930130020311
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 2 p.311 ~ p.320
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A Study of the DNA Extraction from Bloodstain Samples Using Chelex 100
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임찬빈
이존기/김종완/권계철/구선회/박종우/송만수
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Abstract
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Recently genetic variation at the DNA level has been used for the forensic personal identification, and especially polymerase chain reaction(PCR) is useful because of using small quantity of specimen. But the application of PCR technique in the
forensic
field is apt to fail and the destruction of small quantities of DNA is common because there are a lot of the inhibitors of PCR in the circumstances of specimens. Procedures utilizing Chelex(r) 100 resin have been developed to extract DNA from
forensic
samples for use with PCR. Chelex is a chelating resin that has a high affinity for polyvalent metal ion. The Chelex resin is composed of styrene divinylbenzene copolymers containing paired iminodiacetate ion, which act as chelating group. The
purpose of
this investigation was to compare the DNA extraction effeciency of the Chelex method with that of the phenol-chloroform extraction method. The Chelex method was used with amplification and typing of HLA-DQ A genotypes from many different types of
bloodstain samples, including various kinds of fabrics, samples exposed to sunlight, and samples with chemical and microbiological contaminations. The DNA extraction method using Chelex in forensic samples is simple, rapid, safe, and provides
good
results.
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KEYWORD
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